Part:BBa_K1357003:Experience

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Applications of BBa_K1357003

Crystal Violet Assay

Mechanism of the assay: Crystal violet binds to polysaccharides of the biofilm matrix. By measuring the binding to adhering biofilm, the amount of biofilm produced can be quantified. A stock solution of crystal violet is incubated with the culture, the supernatant is removed, bound crystal violet is mobilised with an acetic acid solution and the absorption is determined. The absorption values determine the amount of biofilm present in the culture at the time of staining.

Results of Assay

The biofilm formation rate appears to be following a similar pattern for both the nhaR BioBrick and for the control. However, the nhaR BioBrick has the highest biofilm formation rate between 24 and 48 hours.

User Reviews

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